Single run sequencing (one primer reaction)
 Single run sequencing provides the researcher with the possibility to submit DNA for one primer reactions, using universal or specific primers in a 'single run' sequence which usually provides 700-950 bases of readable sequence.
DNA Sequencing reactions are carried out using Taq DNA polymerase and fluorescently labelled dideoxy terminators and then run on our automated sequencers. Control reactions are carried out to insure high quality control. Single run sequencing reactions can yield 700 to 950 bases routinely, depending on the type of template and primer which are used. Sequences obtained with an automated sequencer begin roughly 15 to 20 bases after the primer sequence. If the sequence of the primer or bases immediately following are required, the template should be sequenced with a reverse primer. Templates for single run sequencing can be purified DNA, bacterial clones, or PCR products. Upon request sequencing reactions can be check for ambiguous nucleotides (N) that the sequencer cannot resolve and are corrected, where possible.
We perform several types of sequencing services:
Customers should be aware of the fact that single read reactions will contain ambiguities and mistakes. Single run sequencing reactions are not publication quality data.
To increase the reliability of the sequence, the template should be sequenced on both strands.
|
