Our collection of almost 2,000 polyclonal antibodies generated against uncharacterized human transmembrane or secreted proteins. The antibodies were made at Primm facility using recombinant protein antigens produced from genes coding for putative transmembrane/secreted proteins.
The human genome codes for almost 10,000 proteins which can be either secreted or membrane associated. Of those, approximately 2,000 are still poorly characterized or unknown proteins. Primm has isolated the cDNA coding for each of those 2,000 proteins, expressed them as His Tag fusions and used the recombinant antigen to generate mouse polyclonal antibodies.
Thanks to the availability of our mouse polyclonals, it is possible to study the function of those proteins in several biological systems using techniques like Immunohistochemistry (IHC) or Western detection. Interestingly, mouse polyclonals are ideal reagents for IHC application due to the very low background of the mouse serum on human cells and tissues.
You can now access our list of mouse polyclonals and purchase those of interest for your research work.
Visit www.yomics.com where an innovative search engineallows you to select the antibody you need
Different from "traditional" proteomic approaches on protein isolation and mass spectrometry characterization, PRIMM's proteomic strategy relies on protein identification by immune-chemistry analysis.
The availability of a "draft" of the entire human genome sequence allows the physical isolation of most of the human genes by Polymerase Chain Reaction (PCR).
Once isolated, each gene is expressed, the corresponding recombinant protein is purified and used to immunize mice to obtain specific polyclonal antibodies.
The availability of a library of sera against a large panel of human proteins is the key feature of the PRIMM technology.
In fact, antibodies can be used to visualize the corresponding proteins, exploiting classical immunochemistry techniques.
If such analysis is carried out comparing protein expression in tissues derived from healthy and disease-carrying individuals, disease-associated proteins can be uncovered.
