Custom Monoclonal Antibody Service

Primm has a very efficient monoclonal antibody production service using mice/rats immunized with different antigens (proteins and peptides) either provided by the customer or produced by Primm. Monoclonal antibodies are important for their high value, for their monospecificity in fact they have affinity for the same epitope and specificity for a unique region of the protein. They could block peculiar protein function and they are very useful tools in biochemistry, molecular biology and medicine. As antigen the best choice are proteins. Proteins can be supplied by the costumer or produced by Primm as HIS-Tag protein fusion (either full length or domains), Primm also uses synthetic peptides even if they are not the ideal immunogen, because they are too small and they wouldn’t allow to generate a large number of monospecific clones. On average 2-3 mg of antigen are necessary for immunization and analysis. This service includes the generation of monoclonal antibodies through somatic fusion and the possibility to choose either 2 or more hybridoma after the first screening to isolate final clones through a sub-cloning by limiting dilution. We can provide both the cell lines and the purified monoclonal Abs. Upon request a cell banking service is available for the storage of the desired clones.

Monoclonal antibodies generation:

• Antigen choice
• Immunization to obtain polyclonal response
• Splenectomy with spleen cells isolation (lymphocytes)
• Splenocytes fusion with myeloma cells
• Selection of positive hybridoma cell lines and isolation of mother clones
• Expansion and sub-cloning by limiting dilution
• Isolation of final clones
• Production (in vivo / cell culture)
• Immunoaffinity-purification of MAbs