Peptide Labeling And Modifications

In addition to our standard peptide synthesis service, Primm offers several peptide modifications for a variety of applications. According to individual needs, we can produce C-amidated, N-acetylated, biotin labeled, fluorescein-labeled, phosphorylated, phosphorylated and cyclic peptides, D-amino acids and MAPs.
Linear peptides can also be bound to a solid support like sepharose for affinity purification of antigen specific polyclonal antibodies (see antibody purification section). Sepharose-peptide affinity columns can also be used to study interactions between immobilized peptide and its ligand or between antisense peptide and the immobilized sense peptide.

Peptide labeling:

Either at the N-term or at the side chain of Lysine residues wherever along the sequence

CHROMOGENIC PROBES:

· Red region of the spectrum

Lissamine

5(6)-carboxytetramethyl-rhodamine [5(6)-TAMRA]

· Green region of the spectrum

5(6)-carboxyfluorescein [5(6)-FAM] or 5-carboxyfluorescein [5-FAM]

4-chloro-7-nitrobenzofuran [NBD]

· Blue region of the spectrum

5-dimethylamino-1-naphtalenesulphonyl [DNS]

FRET PROBES:

· Fluorophores

MCA, Lys(MCA), Glu(Edans)

· Quencher

DABCYL, Lys(DABCYL), DNP, Lys(DNP)

Peptide modifications:

· N-terminal acetylation

·           N-terminal acetylation

· C-terminal amidation

· Linkers: Aminohexanoic Acid/8-Amino-3,6-dioxaoctanoic Acid

· Phosphorylated amino acids (Phosphopeptides)

· D-amino acids

· Methylated peptides

· Formylation

· Bromoacetylation

· Myristoylation

· Succinylation

· Cyclic peptides: Lactam (amide bond formation), Disulfide (S-S bond formation)

· Sepharose-bound peptides

· Conjugated peptides (to carrier protein OVA, KLH or BSA)

SPECIALS:

· 4 branched MAP resin

· Metal chelators for “in vivo” imaging

· Isotope labeling (stable isotopes for biomolecular NMR)