Full Sequencing Projects
Do you need a complete sequence of a plasmid insert or PCR fragment, which is longer than the average of a single read length (700-950 bases)?
Primm strongly recommend ordering its Primer Walking service that can help you to perform your full sequencing project delivering the information you need.
What Full Sequencing Project is:
Primm studies and performs the consecutive design of new primers to extend into unknown sequence and realizes the project to sequence DNA fragments between 1.3 and 10 kilobases, the customer can only submit different type of templates such as:
- purified DNA
- bacterial clones
- PCR products
Primm’ Primer Walking service includes:
- Custom primer design and synthesis
- Single or double stranded sequencing coverage
- Sequence detection on ABI PRISM* 3730xl DNA sequencers
- Assembly of the sequences on the basis of Q30
- Manual editing on sequence chromatograms
- Consensus sequence of assembled data
- Guaranteed accuracy of final data > 99.9%
- Check of a reference sequence
- Re-ordering option within 2 months
- Free storage of template DNA and primers for 2 months
Single strand - Laboratory grade quality
Single strand sequencing provides an overview of the clone sequence, starting from one or two initial reactions using our universal primers or primers indicated by the customer. Based on the resulting sequence data, new primers are designed and synthesized to perform the next run of sequencing reactions. This process is repeated until the entire region on one strand has been covered. Our in-house DNA synthesis production site enables us to offer you the fastest possible turnaround times.
Double strand - Publication grade quality
To submit sequences in a higher accuracy for publication in GenBank or other scientific journals, we strongly recommend to sequence both strands of DNA using our Double Strand sequencing service. Starting from sequencing with a couple of primers we proceed with selecting new ones derived from the ends, ‘till the whole insert/fragment is sequenced on both strands. Our in-house DNA synthesis production site enables us to offer you the best and fastest possible delivery times.
In both service our skilled staff aligns the contigs and the customer receives the assembled sequence and each single electropherogram.
Please inquire for further details about submission requirements.